The Best Binding of Isaac Seeds (Repentance and Afterbirth+)

There’s nothing more enjoyable than happening upon a great BOI seed that gives you OP tears from the outset and allows you to obliterate your foes. Luckily, some people take note of their seeds so that others can enjoy them, too. 

Seeds change depending on the console and the version you’re playing, but we’ve scoured the net and have tried to find the best Repentance and Afterbirth+ seeds. So, here are the best Binding of Isaac seeds available right now, with what to expect in each run. The items found in some of these seeds may change slightly depending on which items you have unlocked. Use the contents menu to skip to the relevant section for you. 

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The Best Binding of Isaac Repentance Seeds

As the most recent DLC, it was harder to find a lot of the best Binding of Isaac seeds for Repentance, but there are still plenty to be getting on with. As of time of writing, Repentance is only available for the PC, so all the Repentance seeds below are only for PC. 

N62G K7JW

Mode: Normal

Items: Ankh, Bible, Tech X, Mutant Spider, Guppy’s Collar, Sausage, Faded Polaroid, Flat File.

Notes: Run works well with Azazel. Find the secret rooms to make the most of the run (the Bible is in a secret room on Depths II). 

D4E4 YK0G 

Mode: Normal

Items: Rocket in a Jar, Goat Head, Dead Cat, Soy Milk, Blood Clot.

Notes: Very fun Isaac seed that leads to some interesting synergies, particularly if playing as Azazel. It’s the run that keeps on giving and continues to get stronger the farther you get. 

QN1W 1DDF 

Mode: Normal

Items: Inner Eye, Almond Milk, Brimstone, Pentagram, Brain Worm.

Notes: Very OP run that will leave you with triple Brimstone and homing shots to boot. 

8Z1E 0TAK 

Mode: Normal

Items: Mutant Spider, Proptosis, Brimstone.

Notes: Brimstone is on the 4th floor and the Brimstone is on the 4th floor’s Devil Room. 

NLZE 1YE8 

Mode: Normal

Items: Inner Eye, Mutant Spider, Squeezy

Notes: This is a good seed if you like multiple tears in one shot. It gets good fast and you can tailor it how you like after getting these strong items early on. 

The Best Afterbirth+ Seeds

PC Afterbirth+ Seeds

Here are the best Binding of Isaac seeds for the PC in the Afterbirth+ DLC.

H9FG 81HH

Mode: Normal

Items: Inner Eye, Mutant Spider, 20/20, Tammy’s Head, Multidimensional Baby

Notes: Fun with any character but Tammy’s Head makes Azazel the character of choice in this one.  

HCER LV8R 

Mode: Normal

Items: Brimstone, Mutant Spider, Magic Mushroom, Lost Contact

Notes: Very powerful seed with great projectile protection from shielded tears. Brimstone is in the second floor devil room. 

0E81 79LB 

Mode: Hard

Items: Ludovico Technique, Small Rock, Cricket’s Head, Spider Bite

Notes: A great seed for Azazel as you get Ludo on the first floor. Small Rock is found by bombing the marked rock on the first floor, and Cricket’s Head is in a stone chest on the second floor. 

Nintendo Switch Afterbirth+ Seeds

Here are a few of the best Binding of Isaac Seeds for Switch: 

MZ4L 3QMG 

Mode: Normal

Items: Mutant Spider, Proptosis, Brimstone, 20/20, The Mulligan, Jacob’s Ladder, Spirit of the Night

Notes: One of the most OP seeds I’ve tried. Pyromaniac also turns up at some point which helps the low number of health upgrades.  

0JH0 CR3E

Mode: Normal

Items: Godhead, Book of Sins, Capricorn, Judas’s Shadow, Athame, Guppy’s Collar, Growth Hormones. 

Notes: Best played as Eden in order to get Godhead as one of the starting items. Would still be a strong seed with other characters, though. 

PS4 Afterbirth+ Seeds

Here are some fun BOI Afterbirth+ seeds for the PS4.

H3TY STBK 

Mode: Normal

Items: Guppy’s Paw, Guppy’s Head, Guppy’s Head, Hive Mind, BFF

Notes: If you like Guppy, you’re in for a treat with this seed. Needs to be played as Isaac in order to get Guppy on the first floor. In the curse room, open the 3 Red Chests, take Guppy’s Paw and then touch Guppy’s Head and replace it again for the D6. Re-roll the head into Guppy’s Collar and let the fun commence!

D6XA WL2H 

Mode: Normal

Items: Guppy, Wafer, Inner Eye, Epic Fetus

Notes: Make sure to visit the Dice Room on the first floor!

Get in Touch

Have you tried any of these best Binding of Isaac Seeds? Or perhaps you know of some other BOI seeds that you’d like to share. If so, let us know in the comment section below using the same format where possible (Seed, Mode, Items, Notes). If any of these don’t work properly or are massively different to what was said, please get in touch to say so.  

For any enquiries, contact me at richard@blogofgames.com

To see Repentance in action, check out the great YouTuber Northernlion have a go at it in the video below!